Composite

Part:BBa_K5236019

Designed by: Hening Guo, Yangzihan Chu   Group: iGEM24_BASIS-China   (2024-10-01)
Revision as of 12:20, 2 October 2024 by Diarmuid (Talk | contribs)

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pET28a-pelB-BhrPETase(W229F)-6xHis

This composite part codes for the mutated BhrPETase W229F and expresses the enzyme in the extracellular space of chassis.

T7 promoter: A Strong promoter recognized by T7 RNA polymerase, used to regulate gene expression of recombinant proteins.

Lac operator: operator that can be activated by IPTG, used to control gene expression by lactose or IPTG.

RBS: ribosome binding site.

BhrPETase W229F: This sequence encodes for a mutated BhrPETase W229F and is derived from Escherichia coli.

pelB: The sequence encodes a signal peptide that enables secretory expression of PETase.

6xHis: A label for protein purification

T7 terminator: Terminates transcription.


Fig.1 Constructed Plasmid

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 277
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 187
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 881


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Categories
Parameters
None