Composite

Part:BBa_K5236018

Designed by: Hening Guo   Group: iGEM24_BASIS-China   (2024-10-01)
Revision as of 12:19, 2 October 2024 by Diarmuid (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


pET28a-pelB-IsPETase(M154L)-6xHis

This composite part codes for the mutated IsPETase M154L and expresses the enzyme in the extracellular space of chassis.

T7 promoter: used to regulate gene expression of recombinant proteins.

Lac operator: used to control gene expression by lactose.

RBS: the location to bind ribosome.

IsPETase M154L: This sequence encodes for a mutated IsPETase M154L and is derived from Escherichia coli.

pelB: the sequence codes for signal peptide which helps PETase exit the cell membrane.

6xHis: a tag for the purification of protein which focus in the production of recombinant proteins.

T7 terminator: terminates transcription.


Fig.1 The result of pET28a-pelB-IsPETase(M154L)-6xHis DNA sequencing

Fig.2 Constructed Plasmid

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1005


[edit]
Categories
Parameters
None