Plasmid

Part:BBa_K5322037

Designed by: Xinquan Zhou   Group: iGEM24_NJTech-China   (2024-10-01)
Revision as of 12:18, 2 October 2024 by Casey (Talk | contribs) (→‎Usage and Biology)

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pET29a-J23119-RBS-SoxR-T7-pSoxS-RBS-eGFP-T7

Usage and Biology

In order to explore the expression of SoxR/SoxS oxidative stress promoter at different induction temperatures, we designed plasmid pET29a-J23119-RBS-SoxR-T7-pSoxS-RBS-eGFP-T7 for subsequent experiments.

pET29a-J23119-RBS-SoxR-pSoxS-RBS-eGFP-T7

Figure 1-1 Plasmid pET29a-J23119-RBS-SoxR-pSoxS-RBS-eGFP-T7

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 683
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 552
    Illegal NheI site found at 702
    Illegal NheI site found at 725
    Illegal NheI site found at 1475
    Illegal SpeI site found at 683
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 683
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 683
  • 1000
    COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None