Part:BBa_K5142048

Click Virus

This part is a genetically engineered vaccinia virus (VACV) with modified A27L protein containing three azidophenylalanine residues close to its N terminus. The azido groups serve as the sites for the click reaction of azido and dibenzocyclooctyne (DBCO) groups, which is the reason why this part is named Click Virus. Since A27L distributes on the surface of VACV and DBCO click reaction can occur under physiological conditions without any catalyst, it is quite easy to link any customized DBCO conjugated element onto the surface of Click Virus covalently via click reaction. Depending on the molecules linked, the modified Click Virus can be applied to a wide variety of biological and medical fields, such as targeting delivery of genes, oncolytic virus and vaccine carrier. In summary, Click Virus is a pluripotent chassis allowing rapid development of customized viral particles without further genetical engineering.

Usage and Biology

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]