Part:BBa_K5120018:Design

Pfr Promoter + Barnase Kill Switch

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal prefix found in sequence at 10226
  Illegal suffix found in sequence at 1
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 10226
  Illegal SpeI site found at 2
  Illegal PstI site found at 16
  Illegal NotI site found at 9
  Illegal NotI site found at 5173
  Illegal NotI site found at 10232
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 10226
  Illegal BglII site found at 644
  Illegal BglII site found at 1657
  Illegal BglII site found at 1913
  Illegal BglII site found at 1933
  Illegal BglII site found at 8791
  Illegal BglII site found at 9775
  Illegal BamHI site found at 1146
  Illegal XhoI site found at 714
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal prefix found in sequence at 10226
  Illegal suffix found in sequence at 2
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal prefix found in sequence at 10226
  Illegal XbaI site found at 10241
  Illegal SpeI site found at 2
  Illegal PstI site found at 16
  Illegal NgoMIV site found at 2397
  Illegal NgoMIV site found at 3353
  Illegal NgoMIV site found at 3636
  Illegal NgoMIV site found at 4168
  Illegal NgoMIV site found at 5045
  Illegal NgoMIV site found at 5169
  Illegal NgoMIV site found at 6196
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

Some silent mutations were made to meet the part assembly standards, pEAQ-DEST1 was modified to include a suffix and prefix

Source

The code for pEAQ-DEST1 was taken from NCBI database The gene for the Pfr Promoter comes from Arabidopsis thaliana genome by going 2000 bp upstream of the Pfr gene The gene for Barnase comes from Part:BBa_K1172904 from the iGEM team iGEM13_Bielefeld-Germany

References

iGEM Parts Registry. (n.d.). Part:BBa_K1172904. Retrieved from https://parts.igem.org/Part:BBa_K1172904