Part:BBa_K4839011

UAS (VP64 binding element)

In order to prove our concept, we construct an expression cassette of GAL4 BS-UAS-GFP-pGK-mcherry. The GAL4 BS (GAL4 binding site) can bind with the GAL4 element and the UAS element can bind with the VP64 element. Once the SNIPR receptor recognize and target to the GPC3 receptor of Huh7 or THP-1 cancer cell, it will mediate downstream signal transduction and cut off the GAL4-VP64 factor. The GAL4-VP64 will bind to the GAL4 binding site and trigger the binding of VP64 and UAS element. The UAS promoter then activated by this transcriptional factor and start the transcription. (Figure1)

Figure1. The prove-of-concept of our SNIPR design

[1] Zhu I , Liu R , Wittsten A H ,et al.Design and modular assembly of synthetic intramembrane proteolysis receptors for custom gene regulation in therapeutic cells[J]. 2021.DOI:10.1101/2021.05.21.445218.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

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