Part:BBa_K5255003:Design
MLACS1
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
LACSs (Long-chain Acyl-CoA Synthetases) are A family of enzymes that play a key role in fatty acid metabolism. They are responsible for converting long-chain fatty acids into corresponding acyl-CoA esters, and then participate in the synthesis and catabolic metabolism of fatty acids. (Lu, 2009) Considering the affinity with the target algae, LACS1 derived from Arabidopsis thaliana was selected for comprehensive analysis by semi-rational methods (such as pyruvate scanning, conservative analysis, etc.) and rational methods (such as modifying the substrate binding residues around the pocket, etc.). The most likely mutation was obtained: amino acid No. 277 was mutated to Met, to increase the affinity and catalytic activity of LACS1 to the DHA substrate, thereby increasing the production efficiency of the target product DHA-PC.