Device

Part:BBa_K175044:Experience

Designed by: Tim_Weenink   Group: iGEM09_TUDelft   (2009-10-11)
Revision as of 04:17, 1 November 2009 by Tim Weenink (Talk | contribs) (→‎Applications of BBa_K175044)

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Please enter how you used this part and how it worked out.

Applications of BBa_K175044

Gel electrophoresis of BBa_K175044 in pSB1AK3 backbone. Lane 1: undigested (supercoiled) plasmid. Lane 2: PstI digested part, expected size = 5095bp. Lane 3: PstI and I-SceI restricted part, expected band lenghts are 3204 and 1891bp. Expected lenghts correspond to the bands observed in the gel.

This part was digested with PstI alone and with PstI and I-SceI. The lenght of the entire biobrick was expected to be 5095bp. The second lane in the adjacent image shows that the observed length corresponds to the predicted length. The part was also digested with PstI and I-SceI, theoretically resulting in two bands of 3204 and 1891bp. The resulting bands in lane 3 of the same figure show that these also correspond well.

Also, the sequence was confirmed by sequencing the part in forward and reverse direction.

User Reviews

UNIQ5a8656774406ef91-partinfo-00000001-QINU

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Tim Weenink

Both the restriction site and the anhydroTetracyclin induction of GFP-LVA have been shown to work

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UNIQ5a8656774406ef91-partinfo-00000003-QINU