Device

Part:BBa_K188151

Designed by: shau-yi wu   Group: iGEM09_NCTU_Formosa   (2009-10-19)
Revision as of 20:09, 31 October 2009 by Poiuytrechu (Talk | contribs)

constitutive promoter +RBS+LacI+RBS+LuxR 

 Constitutive promoter (BBa_J23106) always express the downstream genes, LacI and LuxR (BBa_C0062).

LacI can inhibit the activity of the promoter PLac. So that genes on the second device would'nt express. If there is AHL surrounding, LuxR and AHL will form the complex to induce the expression of the promoter PcIIp22(BBa_K145150) on device F. 

 When lactose is added into the medium, lactose binds with lacI (BBa_K091121) repressor and releases the inhibition of B strand, so the PlacI (K091110) promoter starts transcripting GFP-LVA(BBa_K145015). Then, the medium turns into green in Lactose-accession phase.

Example.jpg

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1208
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
n/aconstitutive promoter +RBS+LacI+RBS+LuxR