Composite
CYP-vgb

Part:BBa_K5291034

Designed by: Yuning Wu   Group: iGEM24_BNUZH-China   (2024-10-01)
Revision as of 09:28, 2 October 2024 by Kortybones (Talk | contribs)


pAB1-cypY96F-vgb

VHb, the first discovered bacterial hemoglobin, is a soluble heme-binding protein with a faster rate of oxygen dissociation. It can enhance cell growth, product synthesis and stress tolerance. And CYPY96F, the P450cam mutant with higher alkane oxidation activity. This composite part is the fusion protein of both.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 604
    Illegal BamHI site found at 684
    Illegal BamHI site found at 1513
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 401
    Illegal NgoMIV site found at 569
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None