Part:BBa_K5198006

minimal CMV

Minimal version of the Cytomegalovirus (CMV) promoter for expression in mammalian cells. miniCMV is a shorter version of the full-length CMV promoter that retains core elements of the CMV promoter for basic transcriptional activity.

This variant of miniCMV was originally used as part of a Gal4UAS system (**BBa_K4839011)**, where it was placed downstream of upstream activating sequence (UAS) [1]. Upon binding of Gal4VP64 (**BBa_K3132000)**, the activity of miniCMV is enhanced, driving the expression of downstream genes.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

References

[1] L. Morsut *et al.*, “Engineering Customized Cell Sensing and Response Behaviors Using Synthetic Notch Receptors,” *Cell*, vol. 164, no. 4, pp. 780–791, Feb. 2016, doi: [10.1016/j.cell.2016.01.012](https://doi.org/10.1016/j.cell.2016.01.012).