Coding

Part:BBa_K5242019

Designed by: Jianting Gong   Group: iGEM24_Tsinghua-M   (2024-09-26)
Revision as of 09:11, 2 October 2024 by Gongjt23 (Talk | contribs)

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E2A

The 2A peptides is a small, self-cleaving sequence derived from the foot-and-mouth disease virus (FMDV). The 2A peptide works by causing a ribosome to skip the final glycine of the 2A sequence, leading to a cleavage event that separates the proteins encoded upstream and downstream of the 2A sequence. We use E2A and LV2A to separate two fluorescent proteins and intermediate peptides. The GSG linker is used to link the 2A peptides with upstream peptides.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 27
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None