Part:BBa_K5335000:Design

MS2 CP tandem dimer fusion containing SpyTag tag

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 331
  Illegal BsaI site found at 760
  

Design Notes

No other details

Source

The series capsid protein sequence was artificially designed and synthesized. MS2 capsid subunits' sequence is from bacteriophage MS2.

References

[1] Peabody DS. Translational repression by bacteriophage MS2 coat protein expressed from a plasmid. A system for genetic analysis of a protein-RNA interaction. J Biol Chem. 1990 Apr 5;265(10):5684-9. [2] Biela AP, Naskalska A, Fatehi F, Twarock R, Heddle JG. Programmable polymorphism of a virus-like particle. Commun Mater. 2022 Feb 7;3:7. doi: 10.1038/s43246-022-00229-3. [3] Biela AP, Naskalska A, Fatehi F, Twarock R, Heddle JG. Programmable polymorphism of a virus-like particle. Commun Mater. 2022 Feb 7;3:7. doi: 10.1038/s43246-022-00229-3. [4] 王丽.诺如病毒GⅡ.17型病毒样颗粒的纯化及免疫原性研究.2023.昆明医科大学,MA thesis.doi:10.27202/d.cnki.gkmyc.2023.001033.