Composite

Part:BBa_K5175041:Design

Designed by: Yuhan Zou   Group: iGEM24_HUST-China   (2024-10-01)
Revision as of 08:12, 2 October 2024 by Andre-zeng (Talk | contribs) (→‎References)


T7 promoter-rhlA-rhlB-T7 terminator


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 827
    Illegal PstI site found at 1811
    Illegal PstI site found at 1925
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2310
    Illegal PstI site found at 827
    Illegal PstI site found at 1811
    Illegal PstI site found at 1925
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 176
    Illegal BamHI site found at 736
    Illegal XhoI site found at 912
    Illegal XhoI site found at 2159
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 827
    Illegal PstI site found at 1811
    Illegal PstI site found at 1925
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 827
    Illegal PstI site found at 1811
    Illegal PstI site found at 1925
    Illegal NgoMIV site found at 1052
    Illegal NgoMIV site found at 1773
    Illegal NgoMIV site found at 1886
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

It is a composite component consisting of the T7 promoter, lac operator, T7 terminator, target genes rhlA, rhlB.
rhlA, rhlB are from Pseudomonas aeruginosa.



Source

Pseudomonas aeruginosa

References

[1] PATRAUCHAN MARIANNA A, FLORIZONE C, DOSANJH M, et al. Catabolism of Benzoate and Phthalate in Rhodococcus sp. Strain RHA1: Redundancies and Convergence [J]. Journal of Bacteriology, 2005, 187(12): 4050-63.

[2] JARVIS F G, JOHNSON M J. A Glyco-lipide Produced by Pseudomonas Aeruginosa [J]. Journal of the American Chemical Society, 1949, 71(12): 4124-6.

[3] ZHU K, ROCK CHARLES O. RhlA Converts β-Hydroxyacyl-Acyl Carrier Protein Intermediates in Fatty Acid Synthesis to the β-Hydroxydecanoyl-β-Hydroxydecanoate Component of Rhamnolipids in Pseudomonas aeruginosa [J]. Journal of Bacteriology, 2008, 190(9): 3147-54.