Composite
AlkB-Adh

Part:BBa_K5291033:Design

Designed by: Yuning Wu   Group: iGEM24_BNUZH-China   (2024-10-01)
Revision as of 08:07, 2 October 2024 by Kortybones (Talk | contribs) (→‎References)

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pAB1-alkB2-Rd45-adhA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 902
    Illegal NgoMIV site found at 2433
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1476


Design Notes

It is designed to accelerate the degradation rate by improving the absorbing and hydroxylation of PE monomer.

Source

AlkB2: Pseudomonas aeruginosa
AhdA: Acinetobacter venetianus RAG-1
Rd45: Dietzia sp. DQ12-45-1b

References

[1]Chen, S., et al. (2024). "An NAD(+)-dependent group Ⅲ alcohol dehydrogenase involved in long-chain alkane degradation in Acinetobacter venetianus RAG-1." Enzyme Microb Technol 172: 110343.
[2]Yuan, M., et al. (2020). "Selective Electroenzymatic Oxyfunctionalization by Alkane Monooxygenase in a Biofuel Cell." Angewandte Chemie. 59(23): 8969-8973.
[3]Guo, X., et al. (2023). "Structure and mechanism of the alkane-oxidizing enzyme AlkB." Nat Commun 14(1): 2180.