DNA

Part:BBa_K5443003

Designed by: Nick Coleman, Carmen Hawthorne   Group: iGEM24_MacquarieUni   (2024-09-27)
Revision as of 07:57, 2 October 2024 by Nickcoleman (Talk | contribs)

4-Hydroxyphenylacetate 3-hydroxylase (HpaBC) from E.coli W is an alternative enzyme to Sam5 to catalyse the hydroxylation of 4-coumaric acid to yield caffeic acid as the second step in the vanillin biosynthesis pathway. HpaBC is a two-component flavin adenine dinucleotide (FAD)-dependent monooxygenase that has a broad host range, and acts on a wide range of phenolic compounds. In our vanillin biosynthesis pathway, HpaBC is performing an ‘unnatural’ role in hydroxylating coumarate, since its physiological substrate is 4-hydroxyphenylacetate.

This part builds on the work of earlier iGEM teams, who contributed HpaBC Parts as follows: BBa_K1124011

c3h-reaction.jpg


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None