Coding
ECFP-RIFMO

Part:BBa_K5439003

Designed by: Osvaldo Sanchez, Diego Cota Barocio   Group: iGEM24_TecMonterreyGDL   (2024-09-30)
Revision as of 07:43, 2 October 2024 by Osvaldosan21 (Talk | contribs) (→‎References)


FRET-based system for the detection of rifampicin

FRET-based sensor system for the detection of rifampicin that consists of rifampicin monooxygenase (K4447003), an enzyme that catalyzes the hydroxylation of rifampicin, flanked by two fluorescent proteins: ECFP (BBa_K1159302) as energy donor and mVenus (BBa_K1907000) as an energy acceptor.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1913
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2562

Usage and Biology

Rifampicin (RAMP) is an antibiotic widely used in the treatment of severe bacterial infections such as tuberculosis, meningitis, leprosy, and HIV-associated infections. RAMP residues contaminate water sources, primarily through human excretions (urine and feces) and waste generated by the pharmaceutical industry and animal husbandry. Due to its high solubility and environmental stability, RAMP is not fully removed by wastewater treatment plants, contributing to the development of antibiotic-resistant bacteria (ARB) 3 .

Figure 1. Predicted structure with the best PAE obtained from ColabFold showing ECFP (green), Rifmo (gray) , and mVENUS (yellow).

Selecting Fluorescent Proteins

FRET (Fluorescence Resonance Energy Transfer) is often used in the design of biosensors as it allows for the specific and sensitive detection of biomolecules in a highly specific manner with high sensitivity, without the need to induce a change in the biomolecule. The fluorescence of the acceptor molecule is activated only when both the donor fluorophore and the acceptor molecule are in proximity. This means that any changes in their surrounding environment that affect the distance between them will also impact the fluorescence of the molecule. This mechanism of action enables the detection of changes in the environment, even if they are subtle, without the need to genetically modify the molecule. FRET is a non-radiative process, which means it does not produce any ionizing radiation. This makes this type of biosensor safer to use and handle compared to others. Additionally, they are very sensitive and versatile biosensors, allowing them to detect the presence of a wide variety of biomolecules, as well as changes in the environment. They can detect protein-protein interactions, monitor changes in pH, measure enzyme activity, among others 2 .

Characterization

Gene amplification, Gibson Assembly and Transformation

We performed a Gibson assembly reaction to insert the Rifmo gene (K444703) into the previous iteration of the ECFP_EryK_mVENUS construct (BBa_K4447004). To accomplish this, the Rifmo gene was amplified using PCR with primers designed to recognize its Open Reading Frame (ORF) and add homology arms for recombination with the pET28b(+) vector. These primers also include NcoI and XhoI restriction sites for further validation. The PCR reaction components are detailed in Table 1, which lists the reagents used for amplification, including DreamTaq Polymerase (Thermo Fisher).


Table 1. Components and volumes for the PCR with DreamTaq polymerase protocol.
Reactive Quantity
10X DreamTaq buffer 5 µL
dNTP Mix (10 mM each) 1 µL
IUpstream primer 1 µL
Downstream primer 1 µL
DNA temple 10 pg - 1 µL
DreamTaq Polymerase 0.25 µL
Nuclease-free water To 50 µL
Total volume 50 µL


The results of the PCR amplification were analyzed by electrophoresis, as shown in Figure 2A. A single clear band around 1.5 kb was observed, corresponding to the predicted molecular weight for the Rifmo gene. The absence of additional bands confirmed the specificity of the primers, with no evidence of primer dimers or nonspecific amplifications, indicating that the PCR was successful.

Figure 2. (A) Agarose gel (0.8%) showing the PCR amplification for the Gibson assembly primer validation of IpfF, TjPCs, RifMo, and their respective controls. The marked bands correspond to the expected molecular weight for each gene of 1.5 kb. (B) Agarose gel (0.8%) showing the amplification of the pET28b(+) backbone along with the two fluorescent proteins, ECFP and mVenus, each amplified using specific primers targeting homologous regions for their respective genes. 3000 bp bands correspond to not-amplified sequences in the supercoil form. .


Table 2. Restriction digest conditions
Componets 2-3 fragment assembly Positive control
Total amount of fragments 0.02 - 0.5 pool 10 µL
Gibson Assembly 2X Master Mix 10 µL 10 µL
Nuclease-Free Water To 20 µL 0 µL
Total volume 20 µL 20 µL


E. coli BL21 cells were transformed with the Gibson Assembly product containing the RifMo gene and the ECFP_mVenus construct. After heat shock, the transformed cells were plated on LB agar with kanamycin (50 μg/mL) and incubated overnight at 37°C. Colonies appeared Figure 2, indicating successful transformation and assembly of the RifMo construct.


Figure 3.Bacterial transformation of ECFP_mVENUS with Rifmo in E.coli BL21 in LB agar with kanamycin (50 µg/mL) .

Restriction Enzyme Digestion

Although the appearance of colonies suggested successful transformation, further validation was needed to confirm the assembly of the RifMo gene. A restriction assay was performed using NcoI and XhoI enzymes, which flank the region containing the assembled RifMo gene. An in silico digestion using Benchling predicted two bands: one for the ECFP_mVenus construct and one for RifMo. In the electrophoresis gel Figure 4, a band around 5 kb was observed, corresponding to the ECFP_mVenus construct, and a band around 3 kb, matching the expected size of RifMo (2.9 kb). These results confirmed the successful assembly of the RifMo construct.

Table 3. Restriction digest conditions
Reactive Quantity
Restriction Enzyme 10X Buffer 5 µL
DNA (1 µg/ µL) 1 µL
Ncol restriction enzyme 1 µL
Xhol restriction enzyme 1 µL
BSA (1 µg/ µL) 0.2 µL
Nuclease-free water To 20 µL
Total volume 20 µL


Figure 4. A) In silico prediction of restriction assay, where the patterns of digestions are shown using the enzymes NcoI and XhoI, and the plasmids (1) pET-28b(+) ECFP_RifMo_mVenus, (2) pET-28b(+) ECFP_TjPCs_mVenus, and (3) pET-28b(+) ECFP_IpfF_mVenus. B) Electrophoresis of agarose gel (0.8%) of the restriction assay with Ncol and XhoI of the construct ECFP_mVenus cloned with the genes IpfF, RifMo or TjPCs. .


Protein Expression

After successfully transforming E. coli BL21 with the Gibson Assembly product containing RifMo, the transformants were induced with 0.4 mM IPTG to overexpress the full ECFP_RifMo_mVenus construct at 16°C overnight. Supernatants from selected clones were collected following cell disruption. The samples were analyzed by SDS-PAGE, which revealed a well-defined band around 109 kDa, corresponding to the expected molecular weight of the ECFP_RifMo_mVenus construct Figure 5 ). This band was absent in the control (induced pET-28b(+) without insert), confirming that no other protein of similar size was expressed.

Figure 5. SDS-PAGE gel showing the protein overexpression results of the ECFP_RIFMO_mVenus construct, 109 kDa correspond to the expected molecular weight of the full protein construct. No band of the same molecular weight as the desired proteins was observed in the control sample. .


Validation of our assembled FRET system ECFP_RIFMO_mVENUS

After validating the overexpression of the RifMo construct, the induced E. coli samples were grown on LB plates containing rifampicin to test the response of the FRET system. The concentrations of rifampicin were based on the reported IC50 for E. coli, using 1.25 mg/mL, 0.625 mg/mL, and 0.312 mg/mL (Weinstein & Zaman, 2018). However, as shown in Figure 6, no fluorescence was observed on the plates. This could be due to the limited time window during which the FRET system fluoresced, which may not have aligned with the observation time on the transilluminator.


Figure 6. Validation of the construct with the substrates of the interest (rifampicin) at a different concentrations .

References

[1]. Hoshino, Y., Fujii, S., Hideki Shinonaga, Arai, K., Saito, F., Fukai, T., Hiroyuki Satoh, Miyazaki, Y., & Ishikawa, J. (2009). Monooxygenation of rifampicin catalyzed by the rox gene product of Nocardia farcinica: structure elucidation, gene identification and role in drug resistance. The Journal of Antibiotics, 63(1), 23–28. https://doi.org/10.1038/ja.2009.116

[2]. Wondimeneh Dubale Adane, Bhagwan Singh Chandravanshi, Getachew, N., & Tessema, M. (2024). A cutting-edge electrochemical sensing platform for the simultaneous determination of the residues of antimicrobial drugs, rifampicin and norfloxacin, in water samples. Analytica Chimica Acta, 1312, 342746–342746. https://doi.org/10.1016/j.aca.2024.342746

[3]. Kumar-Verma, A., Noumani, A., Yadav, A. K., & Solanki , P. R. (2023). FRET Based Biosensor: Principle Applications Recent Advances and Challenges. MDPI, 13(8), 1375–1375. https://doi.org/10.3390/diagnostics13081375

[4]. Weinstein, Z. B., & Zaman, M. H. (2018). Evolution of Rifampin Resistance in Escherichia coli and Mycobacterium smegmatis Due to Substandard Drugs. Antimicrobial agents and chemotherapy, 63(1), e01243-18. https://doi.org/10.1128/AAC.01243-18

[5]. Al-Janabi A. A. (2010). In vitro antibacterial activity of Ibuprofen and acetaminophen. Journal of global infectious diseases, 2(2), 105–108. https://doi.org/10.4103/0974-777X.62880
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