Composite

Part:BBa_K5175042:Design

Designed by: Yuhan Zou   Group: iGEM24_HUST-China   (2024-10-01)
Revision as of 07:31, 2 October 2024 by Andre-zeng (Talk | contribs)


T7 promoter-phaZ-T7 terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 985
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 492
    Illegal NgoMIV site found at 693
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

It is a composite component consisting of the T7 promoter, lac operator, T7 terminator, target genes phaZ.
PhaZ encoded by phaZ is a poly(3-hydroxyalkanoate) depolymerase.


Source

phaZ is from Pseudomonas putida.

References

[20] WITTGENS A, TISO T, ARNDT T T, et al. Growth independent rhamnolipid production from glucose using the non-pathogenic Pseudomonas putida KT2440 [J]. Microb Cell Fact, 2011, 10: 80.

[21] LIU H, CHEN Y, ZHANG Y, et al. Enhanced production of polyhydroxyalkanoates in Pseudomonas putida KT2440 by a combination of genome streamlining and promoter engineering [J]. International Journal of Biological Macromolecules, 2022, 209: 117-24.

[22] 尹进, 车雪梅, 陈国强. 聚羟基脂肪酸酯的研究进展 [J]. 生物工程学报, 32(6): 726-37.