Coding

Part:BBa_K5378007

Designed by: pan zhao   Group: iGEM24_SMU-GDMU-CHINA   (2024-09-25)
Revision as of 07:25, 2 October 2024 by Zhaopan (Talk | contribs)


feaR-A81L


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Usage and Biology

Usage and Biology

FeaR is an AraC family regulator that activates transcription of the tynA and feaB genes in Escherichia coli.FeaR-A81L was identified as PEA-specific variantss, with 580-fold induction.In our project, FeaR-A81L binds to phenylacetaldehyde (PAG) and induces the expression from PtynA.

example
Figure 1. CAPTION_HERE

Functional Verification

example
Figure 2. CAPTION_HERE

Reference

[1]Rottinghaus, A. G., Xi, C., Amrofell, M. B., Yi, H., & Moon, T. S. (2022). Engineering ligand-specific biosensors for aromatic amino acids and neurochemicals. Cell systems, 13(3), 204-214.

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Categories
Parameters
None