Part:BBa_K5332003:Design
CMC (arttificial adhesion protein)
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 470
Illegal PstI site found at 673 - 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1444
Illegal PstI site found at 470
Illegal PstI site found at 673 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 470
Illegal PstI site found at 673 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 470
Illegal PstI site found at 673
Illegal NgoMIV site found at 381
Illegal NgoMIV site found at 1806
Illegal NgoMIV site found at 1810
Illegal AgeI site found at 295
Illegal AgeI site found at 1396 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
We contributed an innovative foundational component to the Registry platform: the artificial glucan-binding protein CMC, designated as BBa_K5332002. This component ingeniously integrates the mechanism of glucan-binding proteins with the scaffold protein CipC from Clostridium cellulovorans. Additionally, we incorporated the mCherry fluorescent protein sequence and the outer membrane protein A (OmpA) signal peptide, designing proteins with multiple copy numbers to optimize performance. To enhance the persistence of engineered strain FMK in the gut and its response in expressing anti-inflammatory factors, we designed a novel artificial glucan-binding protein CMC by simulating the natural mechanisms of glucan-binding proteins. CMC acts as a "bridge" between gut microbiota and the intestinal surface, effectively attracting and recruiting beneficial gut probiotics. This design not only promotes stable adherence of the engineered strain in the gut but also significantly enhances its efficacy in alleviating intestinal inflammation and modulating the gut microenvironment.
Source
The core component of CMC, CBMcipc, is derived from the scaffold protein CipC of Clostridium cellulolyticum