Part:BBa_K5115009

hypB

Introduction

The hypB is a subunit of the hox and hyp operonBBa_K5115020.

Usage and Biology

It's used to build up the hydrogenase which reduces the nickel ion. In our experiment, it was constructed into our ribozyme-assisted polycistronic co-expression system BBa_K5115018(ribozyme+RBS+hypB+stem-loop). And then it will eventually make up parts BBa_K5115066(mineral, U module)and BBa_K5115067(mineral, F module), which are used for further experiments.

Sequence and Features

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal XhoI site found at 336
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 25
  Illegal NgoMIV site found at 871
  Illegal AgeI site found at 823
  
• 1000
  COMPATIBLE WITH RFC[1000]