Regulatory

Part:BBa_K5292360

Designed by: iGEM24_TJUSLS-China   Group: iGEM24_TJUSLS-China   (2024-10-02)
Revision as of 07:01, 2 October 2024 by Registry (Talk | contribs)

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MnfaA-24

In order to improve the expression of the PET-degrading enzyme ICCG, we utilized a Markov transfer frequency matrix to modify the H region of the nfaA signal peptide, resulting in the design of the MnfaA signal peptide. The MnfaA signal peptide was designed to optimize the secretion efficiency of ICCG, and was characterized by its fusion with the ICCG-GFP protein. However, during the experiments, the performance of the MnfaA signal peptide did not fully meet the expected improvement. Nevertheless, this design provides valuable reference information for further optimization and adjustment, especially in exploring the influence of signal peptides on protein secretion. Future work will focus on deeper improvements based on these preliminary results, continuing to explore methods to enhance ICCG secretion and expression.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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