Coding

Part:BBa_K257025:Design

Designed by: Romain Bodinier   Group: iGEM09_Paris   (2009-10-29)
Revision as of 08:37, 30 October 2009 by Sboumahdi (Talk | contribs) (→‎Source)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

Fos : second part of the dimere Jun/Fos


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part needs to have silver standard fusion 23 at both N term and Cterm. Indeed, we use it as a passenger in an autotransporter that allows us to present fos at the outer side of the outer membrane of the cell. We designed a protein fusion with : signal peptide Sec-depedent to export fos from cytoplasm to periplasm + the passenger protein (fos)+ the linker and translocator domain of AIDA which will be anchored in the outer membrane.

Source

DNA synthetized in vitro.

References