DNA
PEBP

Part:BBa_K5291050

Designed by: Yuning Wu   Group: iGEM24_BNUZH-China   (2024-10-01)
Revision as of 06:55, 2 October 2024 by Kendy (Talk | contribs) (Usage and Biology)


PE-binding peptide

Plastic-binding peptides are adsorbed to the plastic surface by hydrogen bonding and other intermolecular forces. Considering that PE-binding peptides(PEBP) has not been extensively studied and we did not find the sequence of PEBP in the paper, we decided to predict PEBP sequence by modeling method. We performed machine learning training; then performed special processing and tried to introduce neural networks. Finally, several predictors were given.


Usage and Biology

In degradation module we constructed pAB1-pS-PEBP-PEase aand pAB1-PEBP-GFP to depolymerize PE microplastics and verify the adsorption capacity of PEBP to PE by fluorescence microscopy.

If you want to know more details, please refer to BBa_K5291051BBa_K5291052.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None