Coding

Part:BBa_K5115004

Designed by: Liyue Chen   Group: iGEM24_Fudan   (2024-09-18)
Revision as of 06:53, 2 October 2024 by Shiyi2003 (Talk | contribs)

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hoxY

contributed by Fudan iGEM 2024

Introduction

The hoxY is a subunit of the hox and hyp operon BBa_K5115020.

Usage and Biology

It's used to build up the hydrogenase which reduces the nickel ion. In our experiment, it was constructed into our ribozyme-assisted polycistronic co-expression system BBa_K5115013(ribozyme+RBS+hoxY+stem-loop). And then it will eventually make up parts BBa_K5115066(mineral, U module)and BBa_K5115067(mineral, F module), which are used for further experiment.

Sequence and Features

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 57
    Illegal XhoI site found at 264
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 357
  • 1000
    COMPATIBLE WITH RFC[1000]


References

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Categories
Parameters
None