Part:BBa_K5127017:Design

T7-iadCDE

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

This part is codon optimized for Goldengate assembly (BsaI, BsmBI, BbsI) and expression in E.coli.

Source

N/A

References

Conway, J. M., Walton, W. G., Salas-González, I., Law, T. F., Lindberg, C. A., Crook, L. E., Kosina, S. M., Fitzpatrick, C. R., Lietzan, A. D., Northen, T. R., Jones, C. D., Finkel, O. M., Redinbo, M. R., & Dangl, J. L. (2022). Diverse MarR bacterial regulators of auxin catabolism in the plant microbiome. Nature Microbiology, 7(11), 1817–1833. https://doi.org/10.1038/s41564-022-01244-3

Gordon, S. A., & Weber, R. P. (1951). Colorimetric Estimation of indoleacetic Acid. Plant Physiology, 26(1), 192–195. https://doi.org/10.1104/pp.26.1.192

Salis, H. M., Mirsky, E. A. & Voigt, C. A. (2009). Automated design of synthetic ribosome binding sites to control protein expression. Nature Biotechnology, 27(10), 946–950. https://doi.org/10.1038/nbt.1568