Part:BBa_K5203002

Epidermin

This part is a 21-amino acid antimicrobial peptide that is produced by certain strains of Staphylococcus epidermidis (Götz et al., 2014). Epidermin plays a significant role in the defense mechanisms of bacteria by inhibiting the growth of other Gram-positive bacteria, including pathogenic strains such as Staphylococcus aureus. Like other lantibiotics, epidermin functions primarily by targeting the cell wall biosynthesis of susceptible bacteria, binding to the lipid II precursor and thereby disrupting the formation of peptidoglycan, a critical component of the bacterial cell wall (Malin et al., 2019). The 2024 iGEM team NYC-Empire-State integrated epidermin into a bacteriocin operon. This operon consisted of three other antimicrobial peptides, including nisin, pep5, and lysostaphin, all separated by the AgrC-AgrA linker and activated by the P2 promoter.

References: Götz, F., Perconti, S., Popella, P., Werner, R., & Schlag, M. (2014). Epidermin and gallidermin: Staphylococcal lantibiotics. International journal of medical microbiology : IJMM, 304(1), 63–71. https://doi.org/10.1016/j.ijmm.2013.08.012 Malin, J. J., & de Leeuw, E. (2019). Therapeutic compounds targeting Lipid II for antibacterial purposes. Infection and drug resistance, 12, 2613–2625. https://doi.org/10.2147/IDR.S215070

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]