Coding

Part:BBa_K5201001

Designed by: YAU LI, Shun Lam Wong   Group: iGEM24_HongKong-UCCKE   (2024-09-30)
Revision as of 05:32, 2 October 2024 by Hyperdive (Talk | contribs)

kfiD codons are optimized for overexpression of UDP-glucose-6-dehyrogenase

Characterisation of BBa_K5201001: HongKong-UCCKE

Document BBa_K5201001 short

Biology

kfiD encodes UDP-glucose-6-dehydrogenase (UGDH) from E. coli K5 strain.



Usage and design

UGDH is endogenously expressed in a low level in E. coli, our recombinant plasmid will overexpress UGDH under an inducible promoter (BBa_R0010). We hope that overexpression of kfiD can increase production of Hyaluronic Acid, by converting UDP-glucose to UDP-glucuronic acid, one of the precursors of HA. HA is widely utilized by the cosmetic industry for its significant water absorption and retention properties. And therefore, we want to further implement it for agricultural use.

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