Plasmid

Part:BBa_K197038

Designed by: Jennifer Brophy   Group: iGEM09_Berkeley_Wetlab   (2009-10-25)
Revision as of 18:48, 25 October 2009 by Jennbrophy (Talk | contribs)

BBb - BseRI/AcuI entry vector

This is an entry vector used for housing basic parts before entry into 2AB layered assembly (an assembly scheme developed at UC Berkeley).

[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2472
    Illegal XbaI site found at 333
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2472
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2472
    Illegal XhoI site found at 1486
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2472
    Illegal XbaI site found at 333
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2472
    Illegal XbaI site found at 333
    Illegal NgoMIV site found at 1339
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
origincoE1
resistanceS