Regulatory

Part:BBa_K239006:Design

Designed by: Axel Nystrom   Group: iGEM09_UCL_London   (2009-06-26)
Revision as of 18:27, 24 October 2009 by Axelnystrom (Talk | contribs) (→‎References)

Modified NarK promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Detect and remove possible restriction sites: XbaI, EcoRI, PstI, SpeI, AgeI, BamHI, BglII, NogMIV, NotI and XhoI. No restriction site had to be removed. Sequence starts 3 bp before the first FNR binding site and ends 6 pb after the -10 box. One pb has been changed in order optimise the first FNR binding site.


Source

Raw genomic sequence from biocyc, Escherichia coli K-12, but modified.


References

Bonnefoy V, DeMoss JA (1992). "Identification of functional cis-acting sequences involved in regulation of narK gene expression in Escherichia coli." Mol Microbiol 1992;6(23);3595-602. PMID: 1474901


Engel, P., M. Trageser, and G. Unden. (1991) Reversible interconversion of the functional state of the gene regulator FNR from Escherichia coli in vivo by O2 and iron availability. Arch. Microbiol. 156:463–470.


Green, J., B. Bennett, P. Jordan, E. T. Ralph, A. J. Thomson, and J. R. Guest. (1996) Reconstitution of the [4Fe-4S] cluster in FNR and demonstration of the aerobic-anaerobic transcription switch in vitro. Biochem. J. 316: 887–892.


Spiro, S., and J. R. Guest. (1987) Regulation and overexpression of the fnr gene of Escherichia coli. J. Gen. Microbiol. 133:3279–3288.


Spiro, S., and J. R. Guest. (1990) FNR and its role in oxygen-regulated gene expression in Escherichia coli. FEMS Microbiol. Rev. 75:399–428.


Kolesnikow T, Schröder I, Gunsalus RP. (1992) Regulation of narK gene expression in Escherichia coli in response to anaerobiosis, nitrate, iron, and molybdenum. J Bacteriol. 1992 Nov;174(22):7104-11.