Part:BBa_K5180777

LuxI_LuxR_Plux_rzrz1

This construct integrates the luxI/luxR quorum sensing system to drive the inducible production of the rzrz01 lysis gene in Escherichia coli. Modeled after the quorum sensing system of Vibrio fischeri, this design enables cell-to-cell communication, ultimately triggering cell lysis. When the luxI gene synthesizes the signaling molecule acyl homoserine lactone (AHL), it binds to the LuxR regulator. The LuxR-AHL complex then interacts with the Plux promoter, activating the transcription of the rzrz01 lysis gene. This results in controlled self-lysis of the bacterial cells in response to AHL accumulation.

Images and Figures

Figure 1: Analysis of Dependent Calprotectin Production Figure 1 demonstrates the impact of calprotectin-dependent lysis. Increases in calprotectin concentration result in zinc deficiency, which can then be detected by the ykgMO promoter. When there is a high concentration of zinc, this pathway is suppressed, showing the feedback mechanism of calprotectin in controlling zinc homeostasis.

<figure> Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  Illegal NheI site found at 829
  Illegal NheI site found at 852
  Illegal NheI site found at 2933
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 1076
  Illegal BamHI site found at 3042
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]