Coding

Part:BBa_K5233032:Design

Designed by: Tianshen TANG   Group: iGEM24_HUST-UEVE-UPSaclay   (2024-10-01)
Revision as of 03:46, 2 October 2024 by Qwerty (Talk | contribs) (→‎Design Notes)


tpiA-tpiB-tphC


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 437
    Illegal PstI site found at 1989
    Illegal PstI site found at 2849
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 437
    Illegal PstI site found at 1989
    Illegal PstI site found at 2849
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2278
    Illegal BamHI site found at 1519
    Illegal BamHI site found at 2028
    Illegal BamHI site found at 3017
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 437
    Illegal PstI site found at 1989
    Illegal PstI site found at 2849
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 437
    Illegal PstI site found at 1989
    Illegal PstI site found at 2849
    Illegal NgoMIV site found at 1473
    Illegal NgoMIV site found at 2776
    Illegal AgeI site found at 718
    Illegal AgeI site found at 1635
    Illegal AgeI site found at 2111
    Illegal AgeI site found at 2570
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

tpiA-tpiB-tphC is a transport system which could transport TPA into the body of engineering bacteria. To check the expression and function of this system, we could use the biosensor system described in BBa_K5233030 to detect the presence of TPA

Source

It comes from Comamonas thiooxidans strain S23.

References