Coding

Part:BBa_K5233030:Design

Designed by: Tianshen TANG   Group: iGEM24_HUST-UEVE-UPSaclay   (2024-10-01)
Revision as of 03:39, 2 October 2024 by Qwerty (Talk | contribs) (→‎Design Notes)


tphR-PtphC-EGFP


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 865
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 865
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 604
    Illegal BamHI site found at 772
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 865
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 865
    Illegal NgoMIV site found at 252
    Illegal AgeI site found at 379
    Illegal AgeI site found at 618
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 703
    Illegal SapI.rc site found at 913


Design Notes

 tphR,PtphC and eGFP can form a biosensor system that could detect the presence of TPA. tphR is combined with TPA to form a complex, which could activate the PtphC and express the downstream reporter gene.
 Since wild E.coli has low ability to transfer TPA into its body, it is suggested to  use tpiA-tpiB-tphC transport system described in 	BBa_K5233032 in order to have better effect

Source

PtphC and tphR comes from Comamonas thiooxidans strain S23, EGFP comes from an assay called "FACS-optimized mutants of the green fluorescent protein (GFP) ".

References