Coding

Part:BBa_K5233021:Design

Designed by: Tianshen TANG   Group: iGEM24_HUST-UEVE-UPSaclay   (2024-09-27)
Revision as of 03:30, 2 October 2024 by Qwerty (Talk | contribs) (→‎Design Notes)

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TurboPETase(malE), a designed enzyme that can be secreted into the extracellular space


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 234
    Illegal AgeI site found at 590
    Illegal AgeI site found at 635
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Since PET is extracellular, signal peptide is used here to reach the goal of secrete the enzyme to the extracellular environment. Besides, enhancer sequence is used to amplify the effect of signal peptide.

Source

The TurboPETase comes from an assay called "Computational redesign of a hydrolase for nearly complete PET depolymerization at industrially relevant high-solids loading". The malE comes from maltose/maltodextrin binding periplasmic protein. The enhancer comes from an assay called "Excretory expression of IsPETase in E. coli by an enhancer of signal peptides and enhanced PET hydrolysis".

References

Cui Y, Chen Y, Sun J, Zhu T, Pang H, Li C, Geng WC, Wu B. Computational redesign of a hydrolase for nearly complete PET depolymerization at industrially relevant high-solids loading. Nat Commun. 2024 Feb 15;15(1):1417. doi: 10.1038/s41467-024-45662-9. PMID: 38360963; PMCID: PMC10869840.