Plasmid

Part:BBa_K5180777

Designed by: Daniel Tu   Group: iGEM24_Princeton   (2024-10-02)
Revision as of 03:28, 2 October 2024 by Danielt4science (Talk | contribs)


LuxI_LuxR_Plux_rzrz1

This construct integrates the luxI/luxR quorum sensing system to drive the inducible production of the rzrz01 lysis gene in Escherichia coli. Modeled after the quorum sensing system of Vibrio fischeri, this design enables cell-to-cell communication, ultimately triggering cell lysis. When the luxI gene synthesizes the signaling molecule acyl homoserine lactone (AHL), it binds to the LuxR regulator. The LuxR-AHL complex then interacts with the Plux promoter, activating the transcription of the rzrz01 lysis gene. This results in controlled self-lysis of the bacterial cells in response to AHL accumulation.

Images and Figures

Figure 1: Analysis of Dependent Calprotectin Production

File:Calprotectindependentlysis.jpg
This image shows the analysis of dependent calprotectin production using the quorum sensing system.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 829
    Illegal NheI site found at 852
    Illegal NheI site found at 2933
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1076
    Illegal BamHI site found at 3042
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None