Plasmid

Part:BBa_K243031

Designed by: Freiburg Bioware09   Group: iGEM09_Freiburg_bioware   (2009-10-20)
Revision as of 18:19, 22 October 2009 by Gerrit (Talk | contribs)

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pMA


This plasmid was used by the Freiburg team for construction and cloning of parts according to the BioBrick Assembly standard 25.


Usage and Biology

This plasmid has been used to assemble and clone a variety of nucleotide sequences. As the assembly standard allows it, the creation of fusion proteins is easily achieved. The vector confers resistance towards ampicillin to the E. coli host cell. Since the inserted part is not expressed, toxic proteins can be cloned and combined.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 1988
    Illegal suffix found in sequence at 2038
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1988
    Illegal SpeI site found at 2039
    Illegal PstI site found at 2053
    Illegal NotI site found at 1994
    Illegal NotI site found at 2046
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1988
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1988
    Illegal suffix found in sequence at 2039
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1988
    Illegal suffix found in sequence at 2029
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//plasmid/construction
Parameters
None