Coding

Part:BBa_K5439006:Design

Designed by: Diego Cota Barocio, Ruben Valenzuela Sanchez   Group: iGEM24_TecMonterreyGDL   (2024-09-30)
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FRET-based system for the detection of ibuprofen


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 2306
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1177
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2174
    Illegal BsaI.rc site found at 2959


Design Notes

NcoI and XhoI restriction sites were added at the 5' and 3' ends. There are no scars between genes due to the use of Gibson Assembly.


Source

The original sequence was reported by Murdoch, R. W., & Hay, A. G. (2013). The sequence for BBa_K1159302 was reported by Poëa-Guyon et al. (2013). The sequence for BBa_K1907000 was reported by Nagai et al. (2002).

References

[1]. Murdoch, R. W., & Hay, A. G. (2013). Genetic and chemical characterization of ibuprofen degradation by Sphingomonas Ibu-2. Microbiology (Reading, England), 159(Pt 3), 621–632. https://doi.org/10.1099/mic.0.062273-0

[2]. Poëa-Guyon, S., Pasquier, H., Mérola, F., Morel, N., & Erard, M. (2013). The enhanced cyan fluorescent protein: a sensitive pH sensor for fluorescence lifetime imaging. Analytical and bioanalytical chemistry, 405(12), 3983–3987. https://doi.org/10.1007/s00216-013-6860-y

[3]. Stassi, D., Donadio, S., Staver, M. J., & Katz, L. (1993). Identification of a Saccharopolyspora erythraea gene required for the final hydroxylation step in erythromycin biosynthesis. Journal of bacteriology, 175(1), 182–189. https://doi.org/10.1128/jb.175.1.182-189.1993