Coding

Part:BBa_K5226034:Design

Designed by: Yujiao Yang   Group: iGEM24_SCUT-China-A   (2024-08-30)
Revision as of 02:26, 2 October 2024 by Admin (Talk | contribs) (→‎Design Notes)

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orfZ


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 295


Design Notes

Although this part complies with one of the RFC 10 or Type IIS standards, it is inconsistent with the standards upheld by other basic parts. As a result, the composite component formed by their combination has illegal sites, necessitating a synonymous mutation. Mutate the base pairs at 1131bp,144bp,1149bp,60bp of orfZ from g/c to a/t.Mutate the base pairs at 414bp and 1161bp of ogdA from a/t to c/g.

Source

PCR amplification.Sequence source is from Clostridium kluyveri DSM 555, found through NCBI (https://www.ncbi.nlm.nih.gov/nucleotide/CP000673.1?report=genbank&log$=nuclalign&blast_rank=2&RID=CZT79ZA7016&from=3066402&to=3067674)

References