Coding

Part:BBa_K5233007:Design

Designed by: Tianshen TANG   Group: iGEM24_HUST-UEVE-UPSaclay   (2024-09-28)
Revision as of 02:09, 2 October 2024 by Lambertbai (Talk | contribs)


tpiB, a kind of tripartite tricarboxylate transporter(TTT) family protein


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 457
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 457
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 496
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 457
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 457
    Illegal AgeI site found at 103
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Wild E. coli has low ability to transfer TPA into its body, so we transformed E. coli with plasmids carrying tpiA and tpiB to transport TPA.


Source

It comes from Comamonas thiooxidans strain S23.

References