Coding

Part:BBa_K5226031:Design

Designed by: Yujiao Yang   Group: iGEM24_SCUT-China-A   (2024-08-29)
Revision as of 01:36, 2 October 2024 by Admin (Talk | contribs) (→‎Design Notes)

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4hbD


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Although this part complies with one of the RFC 10 or Type IIS standards, it is inconsistent with the standards upheld by other basic parts. As a result, the composite component formed by their combination has illegal sites, necessitating a synonymous mutation. Mutate the base pairs at 48bp of hbD from a/t to g/c.

Source

PCR amplification.Sequence source is from Clostridium kluyveri DSM 555, found through NCBI （https://www.ncbi.nlm.nih.gov/nucleotide/CP000673.1?report=genbank&log$=nuclalign&blast_rank=2&RID=D0800NU9013&from=3061070&to=3062185）

References