Part:BBa_K243037

pBAD

The cloning vector pBAD carries the RFC25 restriction sites and an ampicilin resistance. It can be used as an expression vector if the inserted BioBrick contains a ribosome binding site. Expression of the protein of interest can be induced by addition of arabinose.


plasMap

Usage and Biology

Protein expression is only enabled when the inserted coding sequence possesses a ribosome binding site and arabinose is added to the cells, rendering this vector useful for cloning and expression of genes toxic for E. coli.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal prefix found in sequence at 2434
  Illegal suffix found in sequence at 3051
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 2434
  Illegal SpeI site found at 3052
  Illegal PstI site found at 3066
  Illegal NotI site found at 2440
  Illegal NotI site found at 3059
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 2434
  Illegal BamHI site found at 2338
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal prefix found in sequence at 2434
  Illegal suffix found in sequence at 3052
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal prefix found in sequence at 2434
  Illegal suffix found in sequence at 3042
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 3106
  Illegal BsaI site found at 3284
  Illegal BsaI site found at 4352
  Illegal SapI site found at 2155
  Illegal SapI.rc site found at 783
  Illegal SapI.rc site found at 2949