Part:BBa_K5127016:Experience

Results

The Lambda Red recombinase proteins was derived from BNDS-China previous plasmid stock, and Aspink protien was derived from 2023 iGEM distribution kit. We used Golden Gate Assembly to construct pRed-Aspink. PCR and Gel Electrophoresis were performed to verify the success in constructing the fragment and backbone of the full plasmid (Figure 2).

Figure 2. The Agarose gel electrophoresis result of the PCR products of pReplace construction. A,B, materials to construct pReplace. C, golden gate assembly result of pReplace construction. The band at 6910bp in (C) indicated the success in plasmid construction.

The plasmid was transformed into E. coli MG1655, with the colonies displaying a pink color, indicating the successful construction and transformation of the plasmid.