Part:BBa_K5293014:Design

pHREAC_eGFP_ER

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 2334
  Illegal EcoRI site found at 4032
  Illegal XbaI site found at 930
  Illegal SpeI site found at 5878
  Illegal PstI site found at 8535
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 2334
  Illegal EcoRI site found at 4032
  Illegal NheI site found at 3243
  Illegal SpeI site found at 5878
  Illegal PstI site found at 8535
  Illegal NotI site found at 5732
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 2334
  Illegal EcoRI site found at 4032
  Illegal BglII site found at 1297
  Illegal BglII site found at 2560
  Illegal BglII site found at 2580
  Illegal BglII site found at 9350
  Illegal BamHI site found at 936
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 2334
  Illegal EcoRI site found at 4032
  Illegal XbaI site found at 930
  Illegal SpeI site found at 5878
  Illegal PstI site found at 8535
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 2334
  Illegal EcoRI site found at 4032
  Illegal XbaI site found at 930
  Illegal SpeI site found at 5878
  Illegal PstI site found at 8535
  Illegal NgoMIV site found at 3044
  Illegal NgoMIV site found at 4202
  Illegal NgoMIV site found at 4728
  Illegal NgoMIV site found at 5604
  Illegal NgoMIV site found at 5728
  Illegal NgoMIV site found at 6755
  Illegal NgoMIV site found at 7346
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  

Design Notes

NA

[1]

Source

NA