Regulatory
PpetE

Part:BBa_K273019:Design

Designed by: Karl Brune   Group: iGEM09_Uppsala-Sweden   (2009-10-18)
Revision as of 12:05, 22 October 2009 by Karl.brune (Talk | contribs) (→‎Source)

PpetE


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

-


Source

Synechocystis sp.PCC6803


All directions 5' -> 3'

Forward primer: CGCgaattcgcggccgcttctagagTCATAGCGGTTGCCCAATC 71,4°C annealing

Reverse primer: GCGctgcagcggccgctactagtaACTTCTTGGCGATTGTATCTATAGGG 69,9°C annealing

References

[http://www.ncbi.nlm.nih.gov/pubmed/2129338 Copper-induced expression, cloning, and regulatory studies of the plastocyanin gene from the cyanobacterium Synechocystis sp. PCC 6803. Plant Mol Biol. 1990 Oct;15(4):633-42]