Coding

Part:BBa_K5291028

Designed by: Haosheng Lin   Group: iGEM24_BNUZH-China   (2024-09-25)
Revision as of 00:57, 2 October 2024 by Kendy (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


nadM: bifunctional nicotinamide-nucleotide adenylyltransferase

NadM encoded by nadM is involved in both de novo and salvage pathways of NAD biosynthesis. Bifunctional nicotinamide-nucleotide adenylyltransferase catalyzes the addition of adenylate from ATP to β-nicotinamide D-ribonucleotide, forming NAD+. We used the nadM gene to maintain intracellular NAD+ levels.


Usage and Biology

In carbon fixation module we constructed pBBR1MCS2-nadM-nadK-pntA-pntB to improve the intracellular NADPH and NADP+ level.

Fig.1 The map of plasmid pBBR1MCS2-nadM-nadK-pntA-pntB.

We successfully cloned the nadM-nadK gene by PCR.

Fig.2 Electropherogram of nadM-nadK PCR gel.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None