Coding

Part:BBa_K197020:Design

Designed by: Gabriela Guzman Lopez Aguado   Group: iGEM09_Berkeley_Wetlab   (2009-10-21)
Revision as of 05:22, 22 October 2009 by JCAnderson (Talk | contribs) (→‎Design Notes)

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strep


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 22
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 22
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 22
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 22
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is in BglBricks Standard. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BglBricks Standard is available at:

[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BglBricks Standard Description Page]

Source

PCR ca998/ca1363R on pBca9145-Bca9494 (1539 bp, EcoRI/BamHI) Sub into pBca9495CA-Bca1144#5 (EcoRI/BamHI, 3039+910, L) Product is pBca9495CA-Bca1363 {Pbad.rbs.pp.ST}

ca998 Forward Sequencing of pSB1A2/pSB1A3 gtatcacgaggcagaatttcag ca1363R Reverse StrepTag ccataGGATCCctgcccagactgCCCTCCCTC


References