Coding

Part:BBa_K197016:Design

Designed by: Gabriela Guzman Lopez Aguado   Group: iGEM09_Berkeley_Wetlab   (2009-10-21)
Revision as of 05:19, 22 October 2009 by JCAnderson (Talk | contribs) (→‎Design Notes)

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{CPG_L2 (circularly permuted OmpG)}


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 258
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is in BglBricks Standard. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BglBricks Standard is available at:

[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BglBricks Standard Description Page]

Source

PCR ca998/O09ig278 on M10086		  		(873, EcoRI/BamHI)
Sub in pBca9523-Bca1144#5           	(EcoRI/BamHI, 2472+1224, L)
Product is pBca9523-B09cpgl2   		{<CPG_L2>}
----
ca998	Forward Oligo 
CCATAGAATTCATGAGATCTTGGACCCAGATGTCTTCTGGCACTC
O09ig278	Reverse Oligo for the removal of stop codon from {<CPG_L2>}
CGTTAGGATCCcgcgctataatctaccggcccttcc

References