Coding

Part:BBa_K197015:Design

Designed by: Gabriela Guzman Lopez Aguado   Group: iGEM09_Berkeley_Wetlab   (2009-10-21)
Revision as of 05:19, 22 October 2009 by JCAnderson (Talk | contribs) (→‎Design Notes)

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{eCPX (circularly permuted OmpX)}


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 189
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 189
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 189
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 189
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is in BglBricks Standard. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BglBricks Standard is available at:

[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BglBricks Standard Description Page]

Source

 
PCR ca998/O09ig282 on M10014 	(477, EcoRI/BamHI)
Sub in pBca9523-Bca1144#5           	(EcoRI/BamHI, 2472+1224, L)
Product is pBca9523-O09ig282   	{<eCPX>}
-------------------------------------
Ca998  	forward oligo 
gtatcacgaggcagaatttcag
O09ig282 	reverse oligo with stop codons removed for {<eCPX>}
ccggtGGATCCAGAGCTTGCAGTACGGCTTT

References