Coding

Part:BBa_K197012:Design

Designed by: Gabriela Guzman Lopez Aguado   Group: iGEM09_Berkeley_Wetlab   (2009-10-21)
Revision as of 05:18, 22 October 2009 by JCAnderson (Talk | contribs) (→‎Design Notes)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

{VirG(IcsA)}


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 330
    Illegal SpeI site found at 777
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 330
    Illegal SpeI site found at 777
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 330
    Illegal SpeI site found at 777
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 330
    Illegal SpeI site found at 777
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is in BglBricks Standard. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BglBricks Standard is available at:

[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BglBricks Standard Description Page]

Source

PCR O09ig302/O09ig301 on S. Flexneri (490bp, gp = frag1)
PCR O09ig300/O09ig303 on S. Flexneri       (986bp, gp = frag2)
----
PCR O09ig302/O09ig303 on mixed frags    (1457bp, EcoRI/BamHI)
Sub in pBca9523-Bca1144#5           (EcoRI/BamHI, 2472+1224, L)
Product is pBca9523-B09ig302         {?VirG AtD?}
----
O09ig302    Forward PCR of {?VirG AtD?} (B09ig302)
AAGTGGAATTCATGAGATCTTCGTCAAATGTCGGTCAGAC
O09ig301    Reverse internal oligo for {?VirG AtD?} (B09ig302)
CTGCCAGCCTCAGGGCGCC
O09ig300    Forward internal oligo for {?VirG AtD?} (B09ig302)
GGCGCCCTGAGGCTGGCAG
O09ig303    Reverse PCR of {?VirG AtD?} (B09ig302)
GTTAGGGATCCGAAGGTATATTTCACACCC


References