Coding

Part:BBa_K197003:Design

Designed by: Patrick Harrigan   Group: iGEM09_Berkeley_Wetlab   (2009-10-21)
Revision as of 05:14, 22 October 2009 by JCAnderson (Talk | contribs) (→‎Design Notes)

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{cl02365 AtD}


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is in BglBricks Standard. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BglBricks Standard is available at:

[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BglBricks Standard Description Page]

Source

Pool O09ig79 through O09ig112, assemble by PCA
PCR O09ig79/O09ig112 on PCA reaction        (854, EcoRI/BamHI
Sub in pBca9523-Bca1144#5                (EcoRI/BamHI, 2472+1224, L)
Product is pBca9523-B09ig79   {?cl02365 AtD?}
----
O09ig79     PCA assembly of {?cl02365 AtD?} (B09ig79)
CCATAGAATTCATGAGATCTGAAAACTCTAACGCTCGTA
O09ig80     PCA assembly of {?cl02365 AtD?} (B09ig79)
CACTGACCAACGCCAGTCATCCACATACGAGCGTTAGAGTTTTCA
O09ig81     PCA assembly of {?cl02365 AtD?} (B09ig79)
ATGACTGGCGTTGGTCAGTGGGGTAACCTGGACTACGGTTACTCT
O09ig82     PCA assembly of {?cl02365 AtD?} (B09ig79)
CAGCGTAGAAGTCAACGTCCATGTTAGAGTAACCGTAGTCCAGGT
O09ig83     PCA assembly of {?cl02365 AtD?} (B09ig79)
GGACGTTGACTTCTACGCTGGTCTGGTTGGTGCTGAAATCGACGT
O09ig84     PCA assembly of {?cl02365 AtD?} (B09ig79)
CAGACCAGCTTTGGTGTTTTCGGTAACGTCGATTTCAGCACCAAC
O09ig85     PCA assembly of {?cl02365 AtD?} (B09ig79)
AAAACACCAAAGCTGGTCTGTTCTTCGGTGCTGGTTCTACCCACT
O09ig86     PCA assembly of {?cl02365 AtD?} (B09ig79)
ATTTTACCGTATTCGTGAGCTTTGTAGTGGGTAGAACCAGCACCG
O09ig87     PCA assembly of {?cl02365 AtD?} (B09ig79)
GCTCACGAATACGGTAAAATCGACTCTAACGACCTCCATGTTGGT
O09ig88     PCA assembly of {?cl02365 AtD?} (B09ig79)
CTTTGATGTTAGAAACACCGTACAGACCAACATGGAGGTCGTTAG
O09ig89     PCA assembly of {?cl02365 AtD?} (B09ig79)
CGGTGTTTCTAACATCAAAGACGTTGCTGCTGTTAACTACGGTAT
O09ig90     PCA assembly of {?cl02365 AtD?} (B09ig79)
GTCACGGTCCTGGTTGGTGTGGGTGATACCGTAGTTAACAGCAGC
O09ig91     PCA assembly of {?cl02365 AtD?} (B09ig79)
ACACCAACCAGGACCGTGACGCTAAACGTACCCTGTGGGTTGGTC
O09ig92     PCA assembly of {?cl02365 AtD?} (B09ig79)
GAGGTAGAGTTGTAACCAGAGGTCTGACCAACCCACAGGGTACGT
O09ig93     PCA assembly of {?cl02365 AtD?} (B09ig79)
TCTGGTTACAACTCTACCTCTTACGACGCTAAAATCACCCAGTTC
O09ig94     PCA assembly of {?cl02365 AtD?} (B09ig79)
GTTGGGTGTAAGCACCTTCCAGGAAGAACTGGGTGATTTTAGCGT
O09ig95     PCA assembly of {?cl02365 AtD?} (B09ig79)
GGAAGGTGCTTACACCCAACTCAACAACGACAAATACTCTATCGA
O09ig96     PCA assembly of {?cl02365 AtD?} (B09ig79)
GAGCCAGTTGAAACCAGCGTACGGTTCGATAGAGTATTTGTCGTT
O09ig97     PCA assembly of {?cl02365 AtD?} (B09ig79)
ACGCTGGTTTCAACTGGCTCCATGTTTCTACCGACTCTATCAACG
O09ig98     PCA assembly of {?cl02365 AtD?} (B09ig79)
GAGAACTGCATGTTACCAACCTGTTCGTTGATAGAGTCGGTAGAA
O09ig99     PCA assembly of {?cl02365 AtD?} (B09ig79)
GTTGGTAACATGCAGTTCTCTACCAAAACCGACAACCAGGACATC
O09ig100     PCA assembly of {?cl02365 AtD?} (B09ig79)
CACGCAGACCGATAGTGCCAACCTGGATGTCCTGGTTGTCGGTTT
O09ig101     PCA assembly of {?cl02365 AtD?} (B09ig79)
TGGCACTATCGGTCTGCGTGGTGGTTACCCGTTCACCGCTGGTTC
O09ig102     PCA assembly of {?cl02365 AtD?} (B09ig79)
GTCACCTTTCAGAGCCATGTTGATAGAACCAGCGGTGAACGGGTA
O09ig103     PCA assembly of {?cl02365 AtD?} (B09ig79)
ACATGGCTCTGAAAGGTGACGTTTCTGCTTCTCACCTGTTCGGTG
O09ig104     PCA assembly of {?cl02365 AtD?} (B09ig79)
TAGAGTTGAGCTTCCGGACGGTTGTCACCGAACAGGTGAGAAGCA
O09ig105     PCA assembly of {?cl02365 AtD?} (B09ig79)
CGTCCGGAAGCTCAACTCTACCTGTCTAACTCTGGTGAAGCTACC
O09ig106     PCA assembly of {?cl02365 AtD?} (B09ig79)
GGTTGTCCAGTTTACCACCACGCAGGGTAGCTTCACCAGAGTTAG
O09ig107     PCA assembly of {?cl02365 AtD?} (B09ig79)
TGGTGGTAAACTGGACAACCTGTTCGGTGTTGGTCTGGGTGTTGA
O09ig108     PCA assembly of {?cl02365 AtD?} (B09ig79)
GGTGGTAGATTTGTTGAGTTGAGCGTCAACACCCAGACCAACACC
O09ig109     PCA assembly of {?cl02365 AtD?} (B09ig79)
AACTCAACAAATCTACCACCTTCGGTGTTTCTTACCAGGGTCAGT
O09ig110     PCA assembly of {?cl02365 AtD?} (B09ig79)
CCAGAAGAAGAAACGTCAGAGTTGTACTGACCCTGGTAAGAAACA
O09ig111     PCA assembly of {?cl02365 AtD?} (B09ig79)
TCTGACGTTTCTTCTTCTGGTATCAACGCTACCCTGAAAGTTAAC
O09ig112     PCA assembly of {?cl02365 AtD?} (B09ig79)
CGTTAGGATCCGAAGTTAACTTTCAGGGTAGCGT

References